Optimization of transformation protocol of B.juncea with A.tumefaciens Год выпуска: 2012Автор: Mirza Sarwar Baig,Jawaid Ahmad Khan and Mohd Fasih AhmadИздательство: LAP Lambert Academic PublishingСтраниц: 76ISBN: 9783659305214Описание The Indian mustard plant Brassica juncea is known as brown mustard and grown for production of oil seed. They are extremely rich in vitamin-A and K. Conventional breeding techniques for trait improvement of plants are time consuming and labor intensive. Genetic engineering and tissue culture techniques are applied to develop high yielding, herbicide tolerant, pathogen-resistant Brassica juncea. In this work Brassica juncea L. (Pusa jaikisan) was transformed to optimize the transformation protocol using the binary vector pBin 19 containing the neomycin phosphotransferase-II (nptII) gene by Agrobacterium mediated genetic transformation. Co-cultivation of Brassica juncea hypocotyls with Agrobacterium tumefacians strain (LBA4404) was done and co-cultivated hypocotyls were cultured on selection medium having kanamycin. The hypocotyls that showed resistance to kanamycin were selected on the basis of their regeneration potential on selection medium.
Похожие книги
Catherine E. Seta, Paul B. Paulus, Robert A. Baron. Effective Human Relations: A Guide to People at Work (4th Edition). – М.: , 0. – 0 с. T. Parthasarathy, B. Dutta, J. A. M. Potters, T. E. S. Raghaven, D. Ray, A. Sen, Bangalore Indian Institute of Science, Indian Statistical Institute, Jawaharlal Nehru Centre for Advanced Scientific re. Game Theoretical Applications to Economics and Operations Research (Theory and Decision Library. Series C, Game Theory, Mathematical Programming, and Operations Research, Vol 18). – М.: , 0. – 0 с. J. S. Shortle, Ronald C. Griffin, D.W. Bromley, B.G. Colby, A. Dinar, G.E. Helfand, R.E. Howitt, K. Segerson, M. Weinberg, R.A. Young. Irrigated Agriculture and the Environment (The Management of Water Resources Series). – М.: , 0. – 0 с. Michael P. Washburn, Stephen B. Jones, Larry A. Nielsen. The Business of Sustainable Forestry Case Study - Nonindustrial Private Forest Landowners: Nonindustrial Private Forest Landowners: Building the Busi ... tainable Forestry; Analyses and Case Studies). – М.: , 0. – 0 с. Scott B. MacDonald, Georges A. Fauriol. The Politics of the Caribbean Basin Sugar Trade. – М.: , 0. – 0 с. Aspatore Books Staff, InsideTheMinds.com, Scott Opsal, Victoria Collins, Howard Weiss, Sanford B. Axelroth, Robert A. Studen, Gilda Borenstein, Josephine Jimenez, Robert G. Morris. Inside the Minds: Leading Wall Street Investors - Senior Investment Advisors from Merrill Lynch, Bank of America, Montgomery Asset Management & More on the Secrets to Successful Investments in a Down Economy. – М.: , 0. – 0 с. Robert B. Miller, Gary A. Williams, Alden M. Hayashi. The 5 Paths to Persuasion: The Art of Selling Your Message. – М.: Warner Business Books, 2004. – 256 с. Stanley B. Block, Geoffrey A. Hirt. Foundations of Financial Management, 10th Edition: Self-Study Software CD-ROM + Powerweb + FREE SG. – М.: , 0. – 0 с. Lawrence B. Sawyer, Mortimer A. Dittenhofer, James H. Scheiner. Sawyer's Internal Auditing: The Practice of Modern Internal Auditing. – М.: Institute of Internal Auditors, Inc., 2003. – 1446 с. George B. Bradt, Jayme A. Check, Jorge E. Pedraza. The New Leader's 100-Day Action Plan. – М.: John Wiley and Sons, Ltd, 2009. – 296 с. Hector Olasolo, with a Foreword by Judge Sir Adrian Fulford, an Introduction by Judge Ekaterina Tren. The Criminal Responsibility of Senior Political and Military Leaders as Principals to International Crimes. – М.: , 2011. – 396 с. Edited by Christian Joerges and Navraj Singh Ghaleigh with a Prologue by Michael Stolleis and an Epi. Darker Legacies of Law in Europe. – М.: , 2011. – 440 с. Vedamurthy K B and Chauhan A.K. Economic Analysis of Milk Marketing in Karnataka State of India. – М.: LAP Lambert Academic Publishing, 2011. – 68 с. Sandhya Sri B. and Arundhati A. Regeneration of multiple shoot induction in Cajanus cajan. – М.: LAP Lambert Academic Publishing, 2013. – 64 с. Milivoj B. Gavrilov,Ivana A. Tosic and Miodrag Rancic. Numerical Methods in Meteorology. – М.: LAP Lambert Academic Publishing, 2014. – 172 с. Kiran Gangaiah0,Ramappa K.B. and Kadakol A.M. Essentials of services Marketing. – М.: LAP Lambert Academic Publishing, 2013. – 264 с. George B. Bradt, Jayme A. Check, Jorge E. Pedraza. The New Leader's 100-Day Action Plan: How to Take Charge, Build Your Team, and Get Immediate Results. – М.: John Wiley and Sons, Ltd, 2015. – 322 с. Образцы работ
Задайте свой вопрос по вашей теме
Контакты
Поделиться
Мы в социальных сетях
Реклама
Отзывы
Роман Хочу Вас поблагодарить!! Курсовую у меня преподаватель принял только у меня у одного из 20-ти человек, которые в моей группе! При всех мне пожал руку и сказал, что в Москве, куда отсылаются наши курсовые, будет моя курсовая оценена минимум на 4!!!Спасибо Вам!!!)) Новый учебный семестр у меня начнется с 1 октября... И будут опять курсовые... И опять будет принимать именно этот дотошный преподаватель!! ЕЩЕ РАЗ СПАСИБО!!))
