Effect of ageing on the transport of glycyl-glutamine in the kidney Год выпуска: 2012Автор: Othman Alghamdi and Nicola KingИздательство: LAP Lambert Academic PublishingСтраниц: 80ISBN: 9783659280504Описание The renal H+/peptide cotransporters (PEPT1 and PEPT2) play important roles in the reabsorption of di- and tri- peptides. Based os structural similarity, PEPT1 and PEPT2 are also capable of transporting a broad range of peptidomimetic drugs including certain angiotensin converting enzyme (ACE) inhibitors, ?-lactam antibiotics, anti-tumor and anti-viral agents. In the present study, the effect of ageing on L-glycyl-L-glutamine (gly-gln) transport was measured using a novel approach involving the use of the fluorescent indicator, 3,3-Dipropylthiacarbocyanine iodide (DiS-C3-(3)) in membrane vesicles isolated from the superficial cortex and outer medulla of two groups of male Wister rats of approximately 6-weeks (young) and 9-months (middle-aged) old. This study showed for the first time that DiS-C3-(3) fluorescence could be used as a reporter of the membrane potential difference in membrane vesicles isolated from different kidney regions and from rats of different ages. Furthermore it...
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Александр, 06.01 С наступившими и грядущими праздниками Вас! Написанная Вами работа принята преподавателем как есть, мне даже пришлось буквально на месте ваять титульный лист. О недочётах она лишь сказала, что...они есть - и не более того. Благодаря Вам мне даже не пришлось сдавать экзамен по этому предмету. Спасибо большое!
