Evaluation of antiviral and cytotoxic activity Год выпуска: 2011Автор: Waqas Ahmad,Muhammad Ashraf and Aqeel JaveedИздательство: LAP Lambert Academic PublishingСтраниц: 108ISBN: 9783846521922Описание The antiviral activity of plants Glyceriza glabra (roots), Phyllanthus amblicus (Fruit), Eugenia jamolana (Leaves), and Moringa oleifera (Leaves) were evaluated against Infectious bursal disease virus (IBDv) in this study. Ethanolic extraction of these plants was carried out by using Soxhlet apparatus. Four dilutions of each extracts viz 100, 50, 25 and 12.5?g/ml were made. Vero cells were infected by identified infectious bursal disease for antiviral assay. Dilutions of these extracts were applied in triplicate manner on Vero cells (infected by IBDv), that are confluent in 96 well cell culture plates. The cytotoxic activity of mentioned plant extracts was carried out by treating the cells by the same dilutions as used in antiviral assay and incubated the both 96 well cell culture plates for four days. After this incubation, viability of cells was determined by MTT [3-(4,5-dimethylthiazol–2-yl)-2,5-diphenyltetrazolium bromide] colorimetric assay. Endpoint of these assays was measured...
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Юлия Добрый день, Юля! Я сегодня защитила диплом после вашего сопровождения на отлично :-) Хотела сказать Вам ОГРОМНОЕ спасибо за помощь! Вы мне помогали с сопровождением курсовых работ на протяжении всего срока моего обучения. Без Вашего участия мне было бы в сто раз труднее, ведь я работаю и времени на сопровождение курсовых и диплома всегда катастрофически не хватало. Так что в том, что я закончила этот университет есть большая доля и вашего участия. Ещё раз огромное Вам за это спасибо! Будьте счастливы и удачи Вам! При случае, буду рекомендовать Вас своим знакомым, кому понадобятся подобные услуги ;-)
