Год выпуска: 2014 Автор: Miten Jain Издательство: LAP Lambert Academic Publishing Страниц: 200 ISBN: 9783659524196
Описание
ST3Gal1 is a sialyltransferase, an enzyme that adds the sugar sialic acid to chains of other carbohydrates. It has an affinity for the disaccharide, Thomsen-Friedenreich Antigen (TF-Ag), a tumor-associated carbohydrate prevalent on many types of tumor cells, and preferentially adds sialic acid to TF-Ag. TF-Ag is involved in tumor cell metastasis. Previous research found that differential up-regulation of ST3Gal1 expression in a nonmetastatic breast tumor cell line in comparison to a related metastatic breast tumor cell line. This research aims to: 1) clone the involved ST3Gal1 cDNA, 2) add it to the metastatic cell line, and 3) determine changes in cell adhesion and metastatic potential in vitro. TF-Ag is in a cryptic form in normal cells due to masking by sialic acid residues, where as in cancer cells it is not masked. TF-Ag binds to molecules on the inside of blood vessels, helping the tumor cells migrate to other organs, thus playing a role in metastasis. Metastatic potential can...
