Год выпуска: 2010 Автор: Daniele Malleo Издательство: LAP Lambert Academic Publishing Страниц: 220 ISBN: 9783838340838
Описание
The main focus of this work is the novel use of impedance spectroscopy as a tool for single cell analysis. Impedance spectroscopy is a non-invasive, label-free, analytical technique that has found widespread use in disparate scientific and technological fields, but, until now, has been a somewhat neglected tool for the analysis of single cells, mainly due to the technological difficulties intrinsic to the fabrication of microelectrodes and the bio-compatible microenvironments within which the cells under study need to be trapped and isolated. The work begins with a comprehensive literature review and an introductory chapter to the theoretical foundations of impedance spectroscopy. A novel device that can perform continuous differential impedance spectroscopic analysis of single cells hydrodynamically captured in an array inside a microfluidic channel is presented. Iridium oxide and polyelectrolyte coatings are studied as novel electrode materials for impedance...
