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Starch Capped Gold Nanoparticles For Catechol Biosensor



Год выпуска: 2013
Автор: Mohit Rawat and Gurjinder Singh
Издательство: LAP Lambert Academic Publishing
Страниц: 84
ISBN: 9783659312106
Описание
Metallic starch capped gold nanoparticles have been synthesized by the reduction of chloroaurate anions [AuCl4]- solution with hydrazine in the aqueous starch and ethylene glycol solution at room temperature and at atmospheric pressure. The characterization of synthesized gold nanoparticles by UV–Vis spectroscopy, Scanning electron microscopy (SEM), X-ray diffraction (XRD) indicate that average size of pure gold NPs is 5-10 nm, they are spherical in shape and are pure metallic gold. Starch capped gold nanoparticles along with tyrosinase enzyme are effectively entrapped within sol-gel matrix for application in catechol biosensor. Enzymatic reaction between tyrosinase and catechol results in the formation of a colored compound which shows characteristic absorbance at 410 nm. Starch@AuNPs based catechol biosensor showed fast response with linearity for catechol sensing from 1 to 5 mM. Effect of buffer pH and its concentration was also studied and results showed drastic changes in...


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Здравствуйте.Я сама доделала все,в содержании добавила кое-что,оформила все правильно,и защитилась 9ого числа на 4!!!:)