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подбор литературы периодические источники литература по предмету

TOXIGENIC BACTERIAL PROTEASES AND THEIR INHIBITORS



Год выпуска: 2011
Автор: Essam Kotb
Издательство: LAP Lambert Academic Publishing
Страниц: 176
ISBN: 9783843391764
Описание
Among 128 proteolytic bacterial isolates, Brevibacterium otitidis, CBS-76 was choosen on the basis of secreted protease. Analysis of purified protease revealed an apparent molecular weight of 47 KDa with 15.2% glutamic acid content. The purified protease showed the maximum activity after 68h at 35?C and pH 7.2. The purified enzyme was inhibited in the presence of EDTA but maximally activated in the presence of sodium arsenate. LD50 on mice was 8 ?g /100 g body weight. Histological study using transmission electron microscope revealed that nuclei of treated mice hepatocytes appeared with inactive heterochromatin and the nucleoli tend to disintegrate. While the cytoplasm contains swollen mitochondria and some destructed mitochondria lost their inner cristae. And fat droplets accumulated in the cytoplasm in the form of empty vacuoles(steatosis). On studying, novel inhibitors derived from common drugs derivatives of saccharin sodium,compound 6 and 9 showed the maximum...


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Здравствуйте. Марина Михайловна огромное вам спасибо, я защитил на хорошо, только они мой доклад даже слушать не стали сразу попросили назвать Цели и задачи и начали вопросы задавать, но я справился. Спасибо огромное!!!