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Molecular Study of Rice Plant Rhizobacteria of Indo-Nepal Border



Год выпуска: 2012
Автор: Umesh P. Shrivastava
Издательство: LAP Lambert Academic Publishing
Страниц: 200
ISBN: 9783659132094
Описание
The present investigation deals with the isolation and characterization of rhizospheric diazotrophic bacteria from the rice fields of border region of India and Nepal. Molecular diversity among different isolates has been established by PCR-based techniques, restriction analysis of 16S rRNA gene, gene sequencing and DGGE. All the isolates were tested for plant growth promoting characteristics (IAA production, phosphate solubilization, and siderophore production) along with N2 fixation. A novel, rapid and simple method for the screening of IAA production plate assay technique was developed during this study. Our findings show the following significant achievements: i) novel and rapid method of detection of IAA, ii) P solubilization and ACCD activity by Microbacterium (ECI-12A), iii) Siderophore production by Agrobacterium (BN-2A) under salinity stress seems to be the first report. iv) Early flowering by inoculation of BN-2A and v) Species of Agrobacterium, Klebsiella, Serratia,...


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