Molecular and Modern Studies on Potato leaf roll virus (PLRV) Год выпуска: 2014Автор: Emad Fathy Mohamed DwidarИздательство: LAP Lambert Academic PublishingСтраниц: 152ISBN: 9783659553448Описание This investigation was carried out to achieve the following: 1. Conducting some molecular studies on PLRV such as : • Extraction of RNA • Reverse transcriptiom reaction • PCR • Purification of PCR product • Cloning of PCR product • Large scale plasmid preparation(Maxi-prep) • Subcloning • Large scale plasmid preparation(Maxi-prep) 2. Using of modern serological methods for rapid detection of PLRV such as: • ELISA • DBIA • TBIA 3. Recognize the intracellular changes induced by the PLRV using Electron Microscopy 4. Production of PLRV-free potato plants.
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Юлия, 30.06 Спасибо большое за работу. Оценка отлично! У комиссии не было ни одного замечания и вопроса.
