IN VITRO MATURATION AND FERTILIZATION OF OOCYTES IN GOAT Год выпуска: 2011Автор: S. A. Masudul Hoque and Prof. Dr. M. A. M. Yahia KhandokerИздательство: LAP Lambert Academic PublishingСтраниц: 72ISBN: 9783844397291Описание In vitro maturation (IVM) and fertilization (IVF) is the basic requirement of in vitro production (IVP) of embryos. The cumulus cells (CCs) surrounding the oocyte plays a key role of cytoplasmic maturation. It supplies nutrients, energy substrates and/or messenger molecules for the development of oocyte. Oocyte collection techniques have a significant role in IVM. Maturation media also plays a vital role on developmental potential of oocytes in IVP procedure. To establish a well-defined medium different serum have been extensively supplemented to the basic medium. Follicular fluid after necessary treatments could also be used as an economic supplement in the culture media (TCM-199).
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