Год выпуска: 2014 Автор: Junaid Raja Издательство: LAP Lambert Academic Publishing Страниц: 116 ISBN: 9783659561344
Описание
In conventional fed batch process development approaches, batch operating parameters are kept constant and only feeding parameters such as feeding time, post-feed concentration are manipulated, whereas any interactions among the batch and fed-batch operating parameters are not evaluated. In this study an attempt was made to study the interaction between fed-batch operating parameter-post feed glucose concentration (A) and batch operating parameters- seeding density (B), temperature (C), and dissolved oxygen concentration (D) by their simultaneous manipulation, as well as the effect of these interactions on cell growth and monoclonal antibody (mAb) production. NS0 cell line producing the mAb’s against carcino-embryogenic antigen (Anti-CEA) was used. The final mAb concentration, viable cell density and integral of viable cell concentration (IVCC) were the responses evaluated. Statistical analysis experimental data showed that parameter (A) and its interaction with batch parameter (B)...