Год выпуска: 2010 Автор: Reza Gheshlaghi,J. Scharer and A. Elkamel: M. Moo-Young Издательство: LAP Lambert Academic Publishing Страниц: 180 ISBN: 9783838324395
Описание
Scientific methods of enhancing gene expression including genomics, applied molecular biology, metabolic engineering, and combinatorial biosynthesis have been sufficiently developed to make a major impact on recombinant protein production. However, it appears that the combination of trial-and-error experimentation and molecular biology are not sufficient to design an optimum strategy to reach the goal. This book provides a thorough coverage of theoretical and design aspects of two engineering methodologies to overcome this shortcoming. Special emphasis was placed on the effect of the fermentation medium components of A.niger on a recombinant protein production. Statistically based experimental designs in a sequential manner were introduced as powerful tools and applied for medium optimization. A detailed metabolic network comprising intracellular compartments, amino acid catabolism and experimental flux measurements of the key metabolites should be developed and used for...