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In vitro propagation of cassava plants



Год выпуска: 2013
Автор: Neama Abd Alla,Hussein Taha and Mohamed Ragab
Издательство: LAP Lambert Academic Publishing
Страниц: 120
ISBN: 9783659379949
Описание
Cassava (Manihot esculenta Crantz), a perennial vegetatively propagated shrub and a member of the family Euphorbiaceae, is adaptable to different types of soils and cultivated under temperature and rainfall conditions varying between 10°C and 40°C and between 900 mm and 2000 mm, respectively. Stem nodes of cassava, American cultivar, were treated with different concentrations of Clorox at different times (10, 20 and 30 % Clorox for 5, 10 and 15 min). The lowest significant contamination and the highest significant survival percentages were recorded with 20% Clorox for 15 min. Sterilized and survival nodal explants were cultured on MS medium supplemented with different concentrations of BA and Kin (0.1, 0.3, 0.5 and 1.0 mg L-1 from each one alone) in combination with 0.05 mg L-1 of NAA. Maximum number of shootlets per explant (5.67) was achieved on MS medium supplemented with 1.0 mg L-1 BA + 0.05 mg L-1 NAA (proliferation medium). The results demonstrated that there were not observed...


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