Год выпуска: 2012 Автор: Arun Kumar De and Dhruba Malakar Издательство: LAP Lambert Academic Publishing Страниц: 96 ISBN: 9783846580202
Описание
The objective of the present experiment was production of goat embryos either by parthenogenetic activation or by in vitro fertilization and derivation of embryonic stem (ES) cell-like cells. Firstly, the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos were investigated. Secondly, effect of cysteamine supplementation in the in vitro maturation (IVM) or culture (IVC) media, or a combination of both in terms of blastocyst production by in vitro fertilization was investigated. Finally, different methods of isolation of ES cell-like cells from in vitro produced embryos were compared and effects of Leukaemia Inhibitory Factor (LIF) and different types of feeder layers on growth of ES cell-like cells were investigated. The undifferentiated ES cell-like cells showed stem cell like morphological features, normal karyotype, expressed stem cell specific surface and intracellular markers and...
Спасибо большое,Лилечка.Я вам очень благодарна за помощь. Желаю вам не хворать. P.S.:а можно я у вас как-нибудь ещё закажу какую-нибудь консультацию?:)