Год выпуска: 2012 Автор: Leonardo J. Solmesky Издательство: LAP Lambert Academic Publishing Страниц: 128 ISBN: 9783659204463
Описание
Human mesenchymal stem cells from bone marrow (hMSC) show self-renewal capacity and multipotency. hMSC are easily isolated based on their adherence to a plastic surface. Another particular feature of hMSC is their ability to survive without serum. This study shows that, in these conditions, hMSC remain arrested at the G0/G1 phase of the cell cycle, keeping their surface markers expression, as well as their proliferative and differentiation potentials, even after prolonged periods. Based on this advantage, the signals involved in hMSC survival, migration, adhesion, proliferation and differentiation were characterized under these conditions by identifying the endogenous expression of receptors to different growth factors and by manipulating their pathways exogenously. These assays demonstrate that BMP and Wnt pathways are involved in survival. In addition, whereas Erk pathway activation promotes proliferation, migration and differentiation to osteoblasts or adipocytes, RA causes a...
