Development Of SSR And MATK Primers For Marama Bean Год выпуска: 2011Автор: Mutsa TakundwaИздательство: LAP Lambert Academic PublishingСтраниц: 124ISBN: 9783845413969Описание Information on genetic variation of breeding materials helps maintain genetic diversity and sustains long term selection gain. Marama bean [Tylosema esculentum (Burchell) Schreiber] occurs naturally in arid, dry parts of Southern Africa and due to the high nutrient value of the seeds and tubers, rich in protein, oil and starch, it is a potential crop for arid areas where few conventional crops can survive. Recently, the development of SSR enrichment techniques has increased the efficiency of SSR characterization in new species. The microsatellite regions of the genome were the main focus and the matK gene was also explored for its potential to carry out a marama bean genetic diversity study. Microsatellite loci were isolated from the marama bean germplasm using a modified FIASCO enrichment technique. Nine marama bean microsatellite libraries enriched for (AAG)7, (GTT)7, (AGG)7, (GAG)7, (CA)10, (CT)10, (TCC)7, (CA)15 and (CAC)7 were created. The matK gene product was found to be half...
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Misha Здравствуйте Марина. С Вашей помощью мы всё сдали на отлично. Большое Вам спасибо.
