Molecular techniques for investigating toxic dinoflagellate species
Год выпуска: 2011 Автор: Layla Hazeem Издательство: LAP Lambert Academic Publishing Страниц: 188 ISBN: 9783846540190 Описание This book investigates the use of different molecular techniques in detecting and enumerating toxic dinoflagellate species. Polymerase Chain Reaction (PCR) and catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH) have been used and optimized to detect and enumerate the target species. Epifluorescence microscopy and flow cytometry were used in this study. Epifluorescence microscope was shown to be superior for the quantification of the target species, although flow cytometer was demonstrated to be a promising technique for developing an automated detection system.
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