Год выпуска: 2012 Автор: Abdalla Elshereef,Hesham El-Enshasy and Sherif Abdeen Издательство: LAP Lambert Academic Publishing Страниц: 176 ISBN: 9783659159367
Описание
This book offers the experiences and improvements made in mammalian cell culture technology. It bridges two scientific cultures, Scientists with no background in cultivation of animal cells and for engineers with a little ground in biology. It was designed to provide you with immediate access to the protocol required every day for animal and mammalian cell lines cultures. It explains the advantages and disadvantages of the methods, different application and gives advice whichever procedure to use. The author supplements the ideas by simple worked examples, making the techniques of scaling up cells by adaptation strategy for pharmaceutical protein production easy to learn. Monitoring cell growth kinetics accompanied by cell metabolism (glucose and glutamine consumption, lactate and ammonia production) and cell imaging established well. It discusses removal of serum, media supplement like insulin and amino acids, osmotic stress and cell morphology. It worth mentioned that,...
