Год выпуска: 2012 Автор: Nishtha Pandey and Savitha Govardhan Издательство: LAP Lambert Academic Publishing Страниц: 60 ISBN: 9783848420773
Описание
RecQ helicase is a member of helicase superfamily2;the enzyme consists of multiple conserved domains viz,the catalytic core domain (helicase domain and RecQ C-terminal domain) and the HRDC domain.Structures of the individual domains for E.coli are available in the PDB but,complete structure of the protein is not available.We have tried to construct the structure of E.coli RecQ protein by homology modeling and docked the protein to a DNA substrate.Molecular dynamics(MD) simulations of RecQ protein and its complex with DNA were carried out to understand the mode of action of protein.MD simulation of RecQ was found to be stable over a 5ns simulation time suggesting that the structure generated by homology modeling is a stable and favourable conformational state.The RecQ-DNA complex structure simulation displayed interesting dynamics suggesting that the protein has inherent flexibility which manifests itself as domain movements upon substrate binding.Our simulation suggests that DNA...
