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Purification and Immobilization of laccase in xerogels



Год выпуска: 2013
Автор: Shagufta Kamal and Mohammad Asgher
Издательство: LAP Lambert Academic Publishing
Страниц: 216
ISBN: 9783659437076
Описание
Project was planned to study the optimization of production medium, kinetic and characterization of native and entrapped laccase from Pleurotus ostreatus. Production of laccase employing Pleurotus ostreatus during dye decolorization was optimized using different media composition.Purified laccase was immobilized in xerogels using various ratios of PTMOS and TMOS. All immobilized enzyme showed better thermostability and mesostability than native laccase. Although the best method of immobilization might differ from enzyme to enzyme,from application to application and from carrier to carrier, depending on the peculiarities of each specific application, criteria for assessing the robustness of the immobilized enzymes remain the same. Industrial immobilized enzymes must be highly active (high activity in a unit of volume, U g–1 or mL–1), highly selective (to reduce side reactions), highly stable (to reduce cost by effective reuse), cost-effective (low cost contribution thus...


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александр
Спасибо вам за консультацию, после нее подготовленную работу защиту сдал на 4.