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HNO ADDUCTS OF HORSE MYOGLOBIN AND HUMAN HEMOGLOBIN



Год выпуска: 2009
Автор: Dmitry Pervitsky
Издательство: LAP Lambert Academic Publishing
Страниц: 108
ISBN: 9783838301587
Описание
The first chapter is a summary of recent publications about the effect of HNO in the cardiovascular system, detection of HNO in vitro, and physiological reactivity of HNO. The second chapter provides synthetic details of a newly developed method of a synthesis of HNO-Mb of high purity (> 95%). The transient absorbance studies of HNO-Mb revealed completely different results than that of other myoglobin adducts such as MbO2 and NO- Mb. Formation of the Mb-Fe(III)/HNO- geminate pair was confirmed by analyses of transient absorbance spectra at 395 nm. In the third chapter rate constants of the reaction of HNO with Mb-Fe(II), Mb- Fe(III), HNO-Mb, and HNO-Mb with oxygen and nitric oxide were determined using the computational software React for Windows. In addition to the computational method, a direct method of comparison of the rates of the reaction of HNO with proteins was developed. In the last chapter synthesis of HNO adduct of hemoglobin is reported. UV-vis, EPR,...


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Елена (Москва)
Научный руководитель тридцать три раза повторил, что диплом после вашего сопровождения написан гораздо лучше, чем у многих одногруппников. За что Вам огромное спасибо!!!