Methodology for Extraction and Purification of ISOMERASE Год выпуска: 2011Автор: Amna Siddique and Shazia KhurshidИздательство: LAP Lambert Academic PublishingСтраниц: 72ISBN: 9783846522530Описание Mutarotases are recently characterized group of enzymes that are involved in anomeric conversions of monosaccharides enhancing rate of metabolism. Mutarotase was extracted and purified from bovine kidney cortex by (NH4)2SO4 precipitation technique, dialysis and gel filtration chromatography. Polarimeter was used for assay of Mutarotase in this present project. Mutarotase increased activity from 0.478 to 1.711 U/ml by purification. It gained 13.4 folds purification at this final step. It is helpful in determining the glucose level and participates in glycolysis and gluconeogenesis. Activities and fold purification of Mutarotase can also be increased by much improved techniques of purification like ion exchange chromatography or FPLC hence enhancing the production of this enzyme.
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Татьяна и Анжелика Юлия, добрый день. Спасибо большое за корректировку. Вроде как приняли работы после вашего сопровождения. В случае, если на предзащите возникнет необходимость корректировки, то мы Вам напишем, а нет, то еще лучше. Спасибо Вам большое. Я знала, что на Вас можно положиться.
