Год выпуска: 2014 Автор: Chia-chi Liu Издательство: Scholars' Press Страниц: 288 ISBN: 9783639710397
Описание
Generation of peroxide groups in proteins exposed to a wide variety of reactive oxygen species requires an initial formation of protein carbon-centred or peroxyl free radicals, which can be reduced to hydroperoxides. Both protein radicals and protein hydroperoxides are capable of oxidizing important biomolecules and thus initiate biological damage. In this study, we investigated the inhibition of protein hydroperoxide formation by ascorbate and GSH in irradiated HL-60 cells. Incubation of HL-60 cells with Asc in the presence of ascorbate oxidase resulted in the accumulation of intracellular Asc. The intracellular Asc levels were lowered by irradiation. Exposure of HL-60 cells to increasing irradiation doses resulted in increasing accumulation of protein peroxides in the cells. A significant decrease in intracellular protein hydroperoxides was noted when the cells were treated with Asc before irradiation. A dose-dependent protective effect of Asc was observed. Asc also provided a...
Здравствуйте Марина! Хочу Вам сказать большое спасибо за диплом, хоть и пришлось его не много подреставрировать под свою организацию, но в целом всё ОК. Я получил высокие оценки за данную работу. Ещё раз большое спасибо!