Год выпуска: 2012 Автор: Stanley Tom Asaku,Ewan Macleod and Frank Norbert Mwiine Издательство: LAP Lambert Academic Publishing Страниц: 80 ISBN: 9783659190933
Описание
This cross-sectional study provides the first evidence on trypanosome species and subspecies in cattle from Karamoja region, northeast Uganda. In a preliminary survey conducted in May 2011, a total of 706 indigenous cattle from seven communal herds were sampled to detect presence of trypanosomes using micro-haematocrit buffy coat, thick film and thin smear film techniques. The overall herds’ prevalence of trypanosomosis was found to be 5.2% (37/706); 11.5% (9/78) in Lokona kraal, 11.0% (15/137) in Lotanyet and 5.9% (13/220) in Natuket, while the other four communal kraals were aparasitaemic. These parasitological results informed second phase sampling design, in which 199 whole blood samples were collected and preserved on FTA® cards for molecular typing. Trypanosoma vivax dominated with a prevalence of 21.1% (42/199) by ITS-PCR, followed by Trypanozoon 15.6% (31/199) by ITS/TBR-PCRs, T. congolense Savannah 1.5% (3/199) and T. simiae 0.5% (1/199). None of the samples amplified for T....
