Год выпуска: 2010 Автор: Manujendra Saha Издательство: LAP Lambert Academic Publishing Страниц: 68 ISBN: 9783843366649
Описание
Analysis of hepatitis B virus (HBV) infection has been difficult due to a lack of suitable culture system. Pseudotyped viruses provide a safe viral entry model because of their inability to produce infectious progeny virus. Here a system has been developed to prepare vesicular stomatitis virus (VSV) pseudotypes bearing various surface proteins of HBV(HBsAg): S, M or L. The infectivities of the psedotypes were determined by quantifying the number of cells expressing green fluorescent protein (GFP). The infectious unit (IU) of the pseudotype samples neutralized with anti-VSV in the absence and presence of anti-HBs S MAbs and titrated on HepG2 cells ranged from 1,000 to 4,000 IU/ml and 200 to 400 IU/ml, respectively. VSV pseudotype sample bearing HBs M antigen showed the highest infectious titer. This infectivity was inhibited by treatment with lactoferrin or sulfated polysachharides. Pretreatment of the cells with trypsin, tunicamycin or DTT also inhibited the...
