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Identification and Evaluation of biomarkers to detect bladder cancer


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Год выпуска: 2015
Автор: Hafsa W. Abbas
Издательство: LAP Lambert Academic Publishing
Страниц: 252
ISBN: 9783659589409
Описание
 Urinary Bladder Cancer (UBC) is the 5th most common cancer in the West. Continuous efforts have been made to develop non-invasive urine-based biomarkers with high sensitivity and specificity that would improve patients’ quality of life, costs and lower the number of cystoscopies. This can then be utilized as part of a multi-biomarker panel to develop a urine test for UBC diagnosis as a single marker is unable to replace current diagnostic invasive tools. Using a (LC-MS/MS) proteomic approach, 8 UBC and 1 normal bladder cell secretomes were analysed to identify secreted proteins that can be potential candidate biomarkers. phorbol 12-myristate 13-acetate (PMA; PKC activator was used to treat whole secretomes and investigate all proteins released by cells whereas secretomes that were treated with the broad-spectrum inhibitor, Marimastat underwent ultracentrifugation to focus on proteins being shedded. Protein quantification was based upon stable isotope labelling by peptide...

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