Год выпуска: 2012 Автор: Waed Alahmad and Mahmoud Alawi Издательство: LAP Lambert Academic Publishing Страниц: 120 ISBN: 9783659127144
Описание
The present work deals with the development and validated simple HPLC/UV/FLD analytical procedure which enable the measurement of some of the most consumed pharmaceuticals in Jordan (Caffeine, Methotrexate, Diclofenac, Glimepiride, Ibuprofen) at concentration level of ?g/L. Separation was on RP-C8 column with 1 ml/min flow rate of 1:1 H2O, acetonitrile with 0.1% trifluoroacetic acid, extraction on C18 cartridges of 200 ml sample volume. Correlation coefficients of the pharmaceuticals calibration were higher than 0.997 using UV detector and 0.996 using FLD detector. Recoveries were ranged between 90% - 110%. The lowest LOD was 0.38 mg/L. The RSD was ranged between 0.15%- and 9.82%. Samples were taken from three STPs in Amman/ Jordan and two hospital outer drains to detect the concentrations of the studied drugs, the highest concentration was found for Caffeine 1187 ?g/L, Methotrexate 699 ?g/L, Glimepiride 90 ?g/L, Ibuprofen 66 ?g/L and Diclofenac 65 ?g/L. The study also focused on the...
