Год выпуска: 2008 Автор: Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays Издательство: Страниц: 332 ISBN: 1402062400
Описание
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than 15 years ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described, with many of these adaptations and variatios currently being used in clinical, diagnostic and academic laboratories across the world. Further, these techniques are being applied at the diagnostic level (e.g. as high throughput testing methodologies to detect minimum residual disease, the presence/bsence of specific pathogens etc), as well as to increase our understanding of fundamental disease processes. Frequently, PCR technicians and specialists limit their understanding of PCR to one particular methodology. However, this approach limits ther appreciation of the range of versatile PCR techniques currently available, techniques that may be applicable and indeed more suitable to their own laboratory situation. This manual aims to provide the reader with a guide to the...