Год выпуска: 2008 Издательство: Страниц: 84 ISBN: 1843396211
Описание
Among the human caliciviruses, noroviruses are the most common cause of acute gastroenteritis in humans. Exposure to contaminated water is considered a significant health risk because of the low minimal infectious dose. Many published molecular methods are based on the detection of noroviruses in stool samples and limited data is available on their limit of detection. This project attempted to develop novel and rapid real-time RT-PCR based methods for the sensitive detection of noroviruses in environmental water samples. The objectives of this project were to (1) develop and evaluate a carbohydrate ligand-binding assay for the purification of noroviruses from reverse transcriptase-polymerase chain reaction (RT-PCR) interfering substances; (2) develop a real-time RT-PCR assay for noroviruses; (3) compare and evaluate previously published and recently developed primer pairs targeting different regions of the norovirus genome in a conventional RT-PCR assay; and (4) use the developed...
