Opto-biochips for Microcytometry Год выпуска: 2010Автор: Sanket GoelИздательство: LAP Lambert Academic PublishingСтраниц: 156ISBN: 9783838389592Описание Microscale fluorescence activated cell-sorting (micro-FACS) is a technology to identify and separate cells. Different elements of the micro-FACS, fabrication of opto-biochips, particle detection and velocity measurement, and flow switching, are studied in the present work. Biochips were fabricated by casting polydimethylsiloxane (PDMS) on the masters fabricated by direct laser-writing, and standard lithography and dry etching. In a different approach, biochips with integrated waveguides and microchannels were fabricated photolithographically on glass substrates using a negative photoresist, SU-8. In these opto-biochips, two excitation waveguides carrying two different wavelengths intersect the microchannel at two points separated and one large output waveguide carry fluorescence signals to a single filtered detector. The velocities of the microparticles were determined by the captured fluorescence from the double peak. Simulations based on beam propagation method...
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