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Improvement of Rice Genotypes Through Molecular Marker Technique



Год выпуска: 2012
Автор: MD Ashrafuzzaman
Издательство: LAP Lambert Academic Publishing
Страниц: 76
ISBN: 9783659159398
Описание
Rice, Oryza sativa (2n=24) belonging to the family Graminae is the staple food for one third of the world’s population and occupies almost one-fifth of the total land area covered under cereals. Genetic diversity plays an important role in plant breeding because hybrids between lines of diverse origin generally display a greater heterosis than those between closely related strains. PCR-based Random Amplified Polymorphic DNA (RAPD) technique was used for assessing genetic diversity and relationships among different rice genotypes. Amplification with RAPD primers produced 46 scorable bands of which 95.65% were polymorphic. The intra and inter-genotype's similarity indices were calculated. Highest genetic distance was found between IR29 and Binadhan-5 and they remain in different cluster. The genotypes remain in different cluster, are much more diverse and their genetic distance will be higher. Thus, RAPD markers offer a potentially simple, rapid and reliable method to evaluate genetic...


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Здравствуйте Марина...огромное спасидо за Вашу работу...диплом после вашего сопровождения защитила на отлично... еще раз спасибо :-)