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Diagnostic Approaches for detection of BVDV Persistency in Dairy Herds


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Год выпуска: 2011
Автор: Arfan Ahmad,Masood Rabbani and Khushi Muhammad
Издательство: LAP Lambert Academic Publishing
Страниц: 140
ISBN: 9783846529270
Описание
 Bovine viral diarrhea is a major disease of cattle which is causing continuous economic losses to the cattle industry primarily due to decreased reproductive performance. Without doubt, direct contact between BVDV persistently infected, and susceptible animals is the most important transmission route of virus. All control programs which are in use in many countries of the world, mainly depend upon the detection of PI animals, eliminating them and preventing their return into the herds. Therefore, in this study diagnostic suitability of ear notch biopsies and serum samples were compared for the detection of PI animals, as well as proficiency of various diagnostic approaches like VI, AC-ELISA, IHC and Real time RT-PCR were evaluated using ear notch biopsies. It is generally recognized, and we have also confirmed in our experiments, that Real time RT-PCR is more sensitive for BVDV detection than the other methods employed in the study period. However, use of Real time RT-PCR for...

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