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Stem cell and growth factor delivery vehicles for cartilage repair


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Год выпуска: 2012
Автор: Abby Sukarto
Издательство: LAP Lambert Academic Publishing
Страниц: 220
ISBN: 9783659229817
Описание
 Co-delivery of embedded growth factor-loaded microspheres and adult stem cells in a hydrogel matrix was studied for its potential as a cell-based therapeutic strategy for cartilage regeneration in partial thickness chondral defects. A photopolymerizable N-methacrylate glycol chitosan (MGC) was employed to form an in situ gel that was embedded with two formulations of growth factor-loaded microspheres and human adipose-derived stem cells (ASC). The polymeric microspheres were used as a delivery vehicle for the controlled release of growth factors to stimulate differentiation of the ASC towards the chondrocyte lineage. Bone morphogenetic protein-6 (BMP-6) and transforming growth factor-b3 (TGF-b3) were delivered at 5 ng/day at highly bioactive states. The ratio of collagen type II to I per cell was significantly higher for ASC chondrogenesis induced by the sustained release of BMP-6 and TGF-b3 in MGC gels than that of non-induced ASC or with soluble growth factor administration in the...

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